Ellipse Fitting demo

3D Digital Pathology: H&E and IHC Image Registration and Integration

Blair Rossetti, Fusheng Wang, Pengyue Zhang, George Teodoro, Daniel Brat, Jun Kong, Dynamic Registration For Gigapixel Serial Whole Slide Images, IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI), pp.424-428, Melbourne, Australia, April, 2017.
Yanhui Liang, Fusheng Wang, Pengyue Zhang, Joel Saltz, Daniel Brat, Jun Kong, Development of a Framework for Large Scale Three-Dimensional Pathology and Biomarker Imaging and Spatial Analytics, The American Medical Informatics Association (AMIA) Joint Summits on Translational Science, Accepted, 2017.

(Top) Diagram of mapping relationships between reference and target image at low and high resolution, respectively; (Middle) Results of high resolution mapping and propagation for a 4096x4096 pixel region of serial GBM sections; A montage of the registered region is presented on the left, and a 3D rendered volume corresponding to the green box is shown on the right; (Bottom) Qualitative evaluation of registration accuracy using random cross sections. Each row in the right panel displays the y-z cross sections at each registration stage for the corresponding cutting plane in the 3D rendered volume in the left.
Results of IHC image registration:
Results of H&E-IHC multi-stain registration:
Results of H&E-IHC multi-stain registration:
Results of H&E-IHC multi-stain registration:
Results of IHC biomarker detection:
3-D visualization of reconstructed vessels and five different populations of biomarkers:
Correlations between necrotic centers and spatial density of hypoxia biomarker. (Left) H&E pathology slide with a typical necrosis (green box); (Right) Hypoxia marker density (red: high; blue: low).